Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds : IX. Quantitative analysis by in situ reflectance spectroscopy

✍ Scribed by R.W. Frei; H. Zürcher; G. Pataki

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 523 KB
• Volume: 45
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)86212-9

✦ Synopsis

Five nucleo derivatives have been chosen for quantitative in sitzc determination by reflectance spectroscopy after two-dimensional separation from a complex mixture by thin-layer chromatography on cellulose. The use of an internal standard to compensate for fluctuations in the chromatographic procedure resulted in an improvement in the reproducibility of data by about 3-4% relative standard deviation. Average accuracies obtained with the method in the analysis of an artificial mixture ranged between 5.1 and 4.0 %. The use of a linear calibration curve of substance to standard ratio plotted. verszc& square root of concentration proved most advantageous. The use of one set of calibration curves for the analysis of mixtures on various days gave an average error of about 16% but was tremendously timesaving. The method was successfully applied to the analysis of nucleo derivatives in an extract of cartilage red bone marrow. l For part VIII cf. ref. 3. ** To whom all inquiries are to be sent, * ** Present adclrcss: Sandoz Ltd.,