Nuclear transport is becoming crystal clear
β Scribed by Alexis S. Madrid; Karsten Weis
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 488 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0009-5915
No coin nor oath required. For personal study only.
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An MsbA deletion mutant ΞC21 that lacks the two C-terminal Ξ±-helices was expressed in__Escherichia coli__strain C41 and purified by metal-affinity and gel-filtration chromatography. Purified ΞC21 retained 26% of the activity of the wild-type ATPase and had a similar binding affinity to fluorescent n