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Deleting two C-terminal α-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP

✍ Scribed by Terakado, Kanako (author);Kodan, Atsushi (author);Nakano, Hiroaki (author);Kimura, Yasuhisa (author);Ueda, Kazumitsu (author);Nakatsu, Toru (author);Kato, Hiroaki (author)

Publisher: International Union of Crystallography
Year: 2010
Tongue: English
Weight: 916 KB
Volume: 66
Category: Article
ISSN: 0907-4449
DOI: 10.1107/s0907444909055504

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Deleting two C-terminal α-helices is eff

Deleting two C-terminal α-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP

✍ Terakado, Kanako (author);Kodan, Atsushi (author);Nakano, Hiroaki (author);Kimur 📂 Article 📅 2010 🏛 International Union of Crystallography 🌐 English ⚖ 916 KB

An MsbA deletion mutant ΔC21 that lacks the two C-terminal α-helices was expressed in__Escherichia coli__strain C41 and purified by metal-affinity and gel-filtration chromatography. Purified ΔC21 retained 26% of the activity of the wild-type ATPase and had a similar binding affinity to fluorescent n