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Nuclear Transplantation in Mammals: The Role of Maturation Promoting Factor (MPF)

✍ Scribed by Jr. J. Fulka; N. Ouhibi; R. M. Moor; J. Fulka

Book ID
115169441
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
151 KB
Volume
29
Category
Article
ISSN
0936-6768

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## Abstract Dejellied __Rana pipiens__eggs were crushed by centrifugation and clear extracts were prepared by further centrifugation at 150,000 Γ— G for 2 h. The extracts were kept at 0Β°C for 2 to 3 weeks. At intervals during the storage period, 80‐nl aliquots were injected into ovarian oocytes to a