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Nuclear magnetic resonance study of the confirmation of nicotinamide-adenine dinucleotide and reduced nicotinamide-adenine dinucleotide insolution

โœ Scribed by Catterall, William A.; Hollis, Donald P.; Walter, Charles F.

Book ID
127254423
Publisher
American Chemical Society
Year
1969
Tongue
English
Weight
489 KB
Volume
8
Category
Article
ISSN
0006-2960

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The two C-4 protons of reduced nicotinamide adenine dinucleotide (NADH) produce an AB NMR spectrum at 100 MHz as well as at 220 MHz. This observation allows an upper limit of 50 sec-l to be placed on the mean rate of interconversion of the two folded forms of NADH invoked to account for the magnetic

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## Abstract The synthesis of [1โ€^15^N]nicotinamide adenine dinucleotide is described. Chemical shift data from ^15^N NMR studies are presented for the pyridine ring nitrogen of labeled NAD and related compounds. The results indicate a ^15^N label in the Nโ€1 position to be highly sensitive to the re