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15N nuclear magnetic resonance studies of [1-15N]nicotinamide adenine dinucleotides

✍ Scribed by Norman J. Oppenheimer; Robert M. Davidson

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
327 KB
Volume
13
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

The synthesis of [1‐^15^N]nicotinamide adenine dinucleotide is described. Chemical shift data from ^15^N NMR studies are presented for the pyridine ring nitrogen of labeled NAD and related compounds. The results indicate a ^15^N label in the N‐1 position to be highly sensitive to the redox‐state of the pyridine moiety, with an upfield shift of over 100 ppm observed upon reduction of NAD^+^ to NADH. The feasibility of conducting ^15^N NMR studies of pyridine nucleotide binding to dehydrogenases is discussed.

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