Nuclear GTP-binding proteins of Swiss 3T3 cells
β Scribed by Shizu Takeda; Hiromi Sugiyama; Shunji Natori; Kazuhisa Sekimizu
- Book ID
- 115921808
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 374 KB
- Volume
- 244
- Category
- Article
- ISSN
- 0014-5793
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## Abstract The binding of a fluoresceinβisothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 ΞΌM labeled insulin approximately 25% of the internalization was insulinβspecifi
The effects of the anti-cancer anthracyclines doxorubicin and daunorubicin on the activity of protein kinase C (PKC) were examined in intact Swiss 3T3 cells. The 2 drugs stimulated the phosphorylation of an 80K phosphoprotein found to be identical to that generated in response to the PKC activator 1