𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Flow cytofluorometric analysis of insulin binding and internalization by swiss 3T3 cells

✍ Scribed by Robert F. Murphy; Scott Powers; Michael Verderame; Charles R. Cantor; Robert Pollack

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
542 KB
Volume
2
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The binding of a fluorescein‐isothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 μM labeled insulin approximately 25% of the internalization was insulin‐specific. The kinetics of endocytosis were contrasted to those of fluorescent derivatives of histone and dextran. In addition, the fusion of endocytic vesicles containing insulin or dextran with lysosomes was detected by measuring the pH‐dependent increase in fluorescein fluorescence caused by the addition of chloroquine. The application of these results to the analysis of growth control by insulin and related hormones is discussed.

📜 SIMILAR VOLUMES

Binding, internalization, and degradatio
Binding, internalization, and degradation of epidermal growth factor by Balb 3T3 and BP3T3 cells: Relationship to cell density and the stimulation of cell proliferation
✍ Kenneth D. Brown; Yun-Chi Yeh; Robert W. Holley 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

## Abstract Epidermal growth factor (EGF) stimulates the growth of both benzo[a]pyrene‐transformed Balb 3T3 cells (BP3T3) and untransformed Balb 3T3 cells. We describe here the binding, internalization, and degradation of [^125^I]‐EGF by BP3T3 cells and 3T3 cells. Binding of [^125^I]‐EGF reaches a

The binding and processing of plasminoge
The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells
✍ Jerry Tobler; Monty Krieger; Robert M. Stroud 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 989 KB

## Abstract The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells was studied using ^125^I‐labeled canine plasminogen. Throughout a 3‐day period, ^125^I‐plasminogen in the incubation medium bound to the cells and was degraded, first to intermediate‐sized macromolecules that were t

Double fluorescent flow cytometric asses
Double fluorescent flow cytometric assessment of bacterial internalization and binding by epithelial cells
✍ Elizabeth C. de Boer; Rob F. M. Bevers; Karl-Heinz Kurth; Denis H. J. Schamhart 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 645 KB

This study describes a new flow cytometric method for assessment of phagocytosis of specific bacteria (bacillus Calmette-Gukrin (BCG) and Eschericbiu coZi) by bladder epithelial cells. The internalization assay consisted of labeling bacteria chemically with fluorescein hthiocyanate (FITC). Subsequen

Insulin binding and processing by H4IIEC
Insulin binding and processing by H4IIEC3 hepatoma cells: Ultrastructural and biochemical evidence for a unique route of internalization and processing
✍ Robert M. Smith; Nan H. Laudenslager; Neelima Shah; Leonard Jarett 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 980 KB

Biochemical and ultrastructural studies of insulin binding and cellular processing by cultured H411EC3 hepatoma cells were performed. Insulin binding and intracellular accumulation were rapid and after 30 min at 37OC, 65% of t h e total cell-associated 1251-insulin was in an acid-stable compartment.

Specific binding, internalization, and d
Specific binding, internalization, and degradation of human recombinant interleukin-3 by cells of the acute myelogenous, leukemia line, KG-1
✍ Thomas G. Gesner; R. Allan Mufson; Christine R. Norton; Katherine J. Turner; Yu 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 745 KB

We have studied the interaction of 35S-labeled recombinant IL-3 with the acute myelogenous leukemia cell line, KG-1. 35S-IL-3 bound to these cells in a time dependent, saturable, and specific manner at 4OC. Scatchard transformation of binding isotherms demonstrated the existence of a small number (2