𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Binding, internalization, and degradation of epidermal growth factor by Balb 3T3 and BP3T3 cells: Relationship to cell density and the stimulation of cell proliferation

✍ Scribed by Kenneth D. Brown; Yun-Chi Yeh; Robert W. Holley

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
852 KB
Volume
100
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Epidermal growth factor (EGF) stimulates the growth of both benzo[a]pyrene‐transformed Balb 3T3 cells (BP3T3) and untransformed Balb 3T3 cells. We describe here the binding, internalization, and degradation of [^125^I]‐EGF by BP3T3 cells and 3T3 cells. Binding of [^125^I]‐EGF reaches a maximum after 45 to 90 minutes incubation at 37°C. In both BP3T3 and 3T3 cells the extent of EGF binding required to stimulate DNA synthesis is density dependent; sparse cultures require a 15‐30% occupancy to elicit a maximal response whereas dense cultures require a 70‐85% occupancy. At physiological concentrations the total binding of [^125^I]‐EGF to 3T3 cells is higher than to BP3T3 cells, and this difference increases at higher cell densities. The rate of degradation of [^125^I]‐EGF is directly proportional to the total [^125^I]‐EGF binding in each cell type. This supports the hypothesis that one cause of the diminished serum requrement of BP3T3 cells is a reduced rate of utilization of serum growth factors.

📜 SIMILAR VOLUMES

Epidermal growth factor and the control
Epidermal growth factor and the control of proliferation of Balb 3T3 and benzo[a]pyrene-transformed Balb 3T3 cells
✍ Kenneth D. Brown; Robert W. Holley 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 614 KB

## Abstract Benzo[a]pyrene‐transformed Balb 3T3 cells (BP3T3) exhibit “normal” growth controls at low concentrations of serum. Epidermal growth factor (EGF) stimulates DNA synthesis and cell division in both Balb 3T3 and BP3T3 cells at physiological concentrations. The growth response of BP3T3 cell

The binding and processing of plasminoge
The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells
✍ Jerry Tobler; Monty Krieger; Robert M. Stroud 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 989 KB

## Abstract The binding and processing of plasminogen by Balb/c 3T3 and SV3T3 cells was studied using ^125^I‐labeled canine plasminogen. Throughout a 3‐day period, ^125^I‐plasminogen in the incubation medium bound to the cells and was degraded, first to intermediate‐sized macromolecules that were t

Flow cytofluorometric analysis of insuli
Flow cytofluorometric analysis of insulin binding and internalization by swiss 3T3 cells
✍ Robert F. Murphy; Scott Powers; Michael Verderame; Charles R. Cantor; Robert Pol 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 542 KB

## Abstract The binding of a fluorescein‐isothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 μM labeled insulin approximately 25% of the internalization was insulin‐specifi

Density-dependent inhibition of growth:
Density-dependent inhibition of growth: Inhibitorydiffusible factors from 3T3- and rous sarcoma virus (RSV)-transformed 3T3 cells
✍ Louise Harel; Gilles Chatelain; Alice Golde 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 497 KB

We recently fractionated, from the culture medium of 3T3 cells, a therrnolabile inhibitory diffusible factor (IDFN) with a molecular weight of about 40,000 daltons, which decreased nucleic acids synthesis of stimulated target 3T3 cells. In the present publication the inhibitory activities of IDFN (p