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Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals

โœ Scribed by Sugahara, Michihiro ;Kunishima, Naoki

Book ID
104478214
Publisher
International Union of Crystallography
Year
2006
Tongue
English
Weight
216 KB
Volume
62
Category
Article
ISSN
0907-4449

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โœฆ Synopsis

Determination of a suitable cryoprotectant agent condition is one of the time-consuming processes in protein X-ray crystallography. The adaptability of two novel versatile cryoprotectants (oil- and water-based types) has been evaluated by cryodiffraction experiments using crystals of five different proteins. The results suggest that complementary use of both oil- and water-based cryoprotectants enables the successful cryoprotection of protein crystals. Since the versatile cryoprotectants do not contain crystallization solution, rapid and efficient preparation of heavy-atom derivatives is possible with these cryoprotectants in the presence of heavy-atom reagents. Four heavy-atom reagents [K(2)PtCl(4), HgCl(2), KAu(CN)(2) and K(2)Pt(CN)(4)] were examined and could be stored with sufficient derivatizing capabilities for over one month in the versatile cryoprotectants.

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Heavy-atom Database System (HATODAS) is a WWW-based tool designed to assist the heavyatom derivatization of proteins. The conventional procedure for the preparation of derivatives is usually a time-consuming 'trial-and-error' process. The present program provides a solution for this problem using a