Heavy-atom Database System: a tool for the preparation of heavy-atom derivatives of protein crystals based on amino-acid sequence and crystallization conditions
✍ Scribed by Sugahara, Michihiro ;Asada, Yukuhiko ;Ayama, Haruhiko ;Ukawa, Hisashi ;Taka, Hideyuki ;Kunishima, Naoki
- Publisher
- International Union of Crystallography
- Year
- 2005
- Tongue
- English
- Weight
- 270 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0907-4449
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✦ Synopsis
Heavy-atom Database System (HATODAS) is a WWW-based tool designed to assist the heavyatom derivatization of proteins. The conventional procedure for the preparation of derivatives is usually a time-consuming 'trial-and-error' process. The present program provides a solution for this problem using a database of known heavy-atom derivatives. A database search suggests potential heavy-atom reagents for any target protein based on its amino-acid sequence and crystallization conditions. A mining of the database identified 93 preferred motifs for heavy-atom binding. The motifs are observed frequently at the actual heavy-atom-binding sites encountered in the process of structure determination.