However, to take full advantage of the biocatalysis lipase complexes has been developed utilizing water in in an organic medium, it is necessary to modify the oil emulsions. In order to optimize the preparation condienzymes to be soluble in an organic solvent from the tions, we have investigated the effects of several operaviewpoint of reduced diffusional limitations of subtional parameters on the enzymatic activity of the surfacstrates and increase in reaction rates.
tant-lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation At least two approaches toward that goal have been conditions [alkaline pH 8-10, organic/aqueous ϭ 90/10 successfully developed. Yoshimoto et al. (1984) first re-(v/v), concentration of surfactant, 10 mM], the surfactantported a modification method for an enzyme with an lipase complex efficiently catalyzed the esterification of synthetic polymer, and several groups conducted further benzyl alcohol with lauric acid in organic media. The esmodification of an enzyme with some polymers. Anterification rate of the surfactant-lipase complex was increased over 16-fold relative to the native powder lipase.
other interesting modification method for enzymes is Furthermore, the lipase complex showed high storage the surfactant-coating method, in which enzymes are stability.