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Novel missense mutation in ALS2 gene results in infantile ascending hereditary spastic paralysis

✍ Scribed by Eleonore Eymard-Pierre; Koji Yamanaka; Martin Haeussler; Wolfram Kress; Fernande Gauthier-Barichard; Patricia Combes; Don W. Cleveland; Odile Boespflug-Tanguy


Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
182 KB
Volume
59
Category
Article
ISSN
0364-5134

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## Detection conditions sequence of primers: F: ACATGGTTAAGGCCTGTTGC R: GCCACATCTGGCTTGAAATT PCR conditions: 3 min at 94oC; 30 cycles of 1 min 94oC, 1 min at 62oC and 1 min at 72oC; 10 min at 72oC electrophoresis: SSCP -10% native polyacrylamide gel (37.5:1 acrylamide: bis-acrylamide), 0.5x TBE,