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Novel enzyme immunoassay (Immune Complex Transfer Enzyme Immunoassay) for anti-thyroglobulin IgG in human serum

✍ Scribed by Takeyuki Kohno; Eiji Ishikawa; Tomohiro Mitsukawa; Shigeru Matsukura

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 496 KB
Volume: 2
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860020406

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✦ Synopsis

A novel enzyme immunoassay (immune complex transfer enzyme immunoassay) for anti-thyroglobulin IgG in human serum is described. Anti-thyroglobulin IgG in human serum was reacted with dinitrophenyl thyroglobulin, and the complex formed between anti-thyroglobulin IgG and dinitrophenyl thyroglobulin was trapped onto an affinity-purified rabbit (anti-dinitrophenyl bovine serum albumin) IgG-coated polystyrene ball. After eliminating nonspecific IgG in test serum by washing, the complex was eluted from the polystyrene ball with dinitrophenyl-L-lysine and transferred to a clean polystyrene ball coated with rabbit anti-thyroglobulin IgG. The amount of hu- man anti-thyroglobulin IgG in the complex on the rabbit anti-thyroglobulin IgG-coated polystyrene ball was estimated using rabbit (anti-human IgG 7-chain) Fab'-horseradish peroxidase conjugate. The present enzyme immunoassay was 1,000-3,000- fold more sensitive than the conventional enzyme immunoassay, in which a thyroglobulin-coated polystyrene ball was incubated with serum containing antithyroglobulin IgG and subsequently with rabbit (anti-human IgG 7-chain) Fab'horseradish peroxidase conjugate. By the present enzyme immunoassay, anti-thyroglobulin IgG was demonstrated in all patients with Graves' disease and chronic thyroiditis.

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