Measurement of anti-thyroglobulin IgG in urine of patients with autoimmune thyroid diseases by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay)

✍ Scribed by Yoshikuni Yogi; Kouichi Hirota; Takeyuki Kohno; Hirotaka Toshimori; Shigeru Matsukura; Toshiaki Setoguchi; Dr. Eiji Ishikawa

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 786 KB
• Volume: 7
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860070112

✦ Synopsis

Anti-thyroglobulin IgG in urine of patients with Graves' disease and chronic thyroiditis and healthy subjects was measured by a sensitive enzyme immunoassay (immune complex transfer enzyme imrnunoassay). Anti-thyroglobulin IgG in dialyzed urine was reacted simultaneously with 2,4-dinitrophenylated thyroglobulin and thyroglobulin-p-o-galactosidase conjugate. The immune complex formed consisting of the three components was trapped onto polystyrene balls coated with (anti-2,4,dinitrophenyl group) IgG, eluted with ~N-2,4-dinitrophenyl-~-lysine, and transferred onto polystyrene balls coated with (antihuman IgG y-chain) IgG. @-D-Galactosi-dase activity bound to the last polystyrene balls was assayed by fluorometry. Anti-thyroglobulin IgG was detected in most of the patients, but not in most of the healthy subjects; levels of anti-thyroglobulin IgG in urine of the patients were well correlated to those in serum of the same patients. The measurement of anti-thyroglobulin IgG in urine by the immune complex transfer enzyme immunoassay was suggested to be useful as a diagnostic aid for autoimmune thyroid diseases. The conventional standard ELISA was not sufficiently sensitive for measuring anti-thyroglobulin IgG in urine. D 1 9 9 3 ~i 1 e y -~i s s .

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