Selection of High-Affinity Binding Sites
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S. Pierrou; S. Enerback; P. Carlsson
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Article
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1995
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Elsevier Science
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English
⚖ 695 KB
We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble