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Novel DNA-binding protein from Drosophila embryos identified by binding site selection

✍ Scribed by Vesna Todorović; Snežana Kojić; Miloš Vujanac; Ana Savić; Dragana Stefanović


Book ID
115932996
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
532 KB
Volume
396
Category
Article
ISSN
0014-5793

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We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble