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In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiae

✍ Scribed by Rasa Beinoravičiūtė-Kellner; Georg Lipps; Gerhard Krauss

Book ID
116465822
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
288 KB
Volume
579
Category
Article
ISSN
0014-5793

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✍ S. Pierrou; S. Enerback; P. Carlsson 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble