Novel cytomorphology of the giant proerythroblasts of parvovirus B19 infection

✍ Scribed by Koduri, Prasad Rao

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 390 KB
• Volume: 58
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/(sici)1096-8652(199806)58:2<95::aid-ajh1>3.0.co;2-v

✦ Synopsis

The morphology of the giant proerythroblasts (GPE) in air-dried and Wright-Giemsastained smears of bone marrow in 16 patients with pure red cell aplasia (PRCA) caused by parvovirus B19 infection is described. B19 infection was diagnosed by the presence of the virus or viral DNA and/or IgM antibodies. Twelve patients had chronic hemolytic anemia and aplastic crisis and 4 patients had AIDS with chronic PRCA. In patients with chronic hemolytic anemia and aplastic crisis, GPE were not detectable in bone marrow biopsies that showed any degree of recovery of erythropoiesis. The GPE morphology was quite variable. The early (basophilic) GPE measured 25 to 35 µm in diameter, had a narrow rim of intensely blue and often vacuolated cytoplasm with pseudopodia, round nuclei with compact uncondensed chromatin, and an indistinct and inclusion-like purplecolored tinctorial change. The ''intermediate'' and ''late'' GPE measured 25 to 45 µm in diameter and showed cytoplasmic swelling, gradual loss of cytoplasmic basophilia, and fraying of the cytoplasm with focal rupture; the nuclei showed an increase in volume, a highly uncondensed and coarse sieve-like chromatin, and 1 to 3 prominent, pale to moderate purple inclusion-like nucleoli or inclusions. Bare nuclei similar in size and chromatin pattern to those of the GPE were present in proximity to the GPE and may have arisen from the GPE by dissolution of the cytoplasm. The glassy intranuclear inclusions with central clearing, the so-called lantern cells described in formalin-fixed tissues of patients with B19 infection, were absent in all cases. These findings suggest that direct toxic cell injury rather than apoptosis may be involved in the pathogenesis of erythroid aplasia in B19 infection. Am.