Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

✍ Scribed by Shanta Raj Bhattarai; Ho-Keun Yi; Narayan Bhattarai; Pyoung Han Hwang; Hak Yong Kim

• Book ID: 103999343
• Publisher: Elsevier Science
• Year: 2006
• Tongue: English
• Weight: 341 KB
• Volume: 2
• Category: Article
• ISSN: 1742-7061
• DOI: 10.1016/j.actbio.2005.10.007

✦ Synopsis

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was signiWcantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the -galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantiWed by -galactosidase assay and spectroXuorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could Wnd application in gene therapy.