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Novel assay for the detection of immunoglobulin G antihuman immunodeficiency virus in untreated saliva and urine

✍ Scribed by Jeffrey A. Connell; John V. Parry; Philip P. Mortimer; Julian Duncan

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
573 KB
Volume
41
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Epidemiological evidence and laboratory studies indicate that human immunodeficiency virus type 1 (HIV 1) is rarely, if ever, transmitted in saliva or urine. In that both specimens are easy to collect, each may be a useful alternative to serum specimens for anti‐HIV screening. A rapid, simple, and robust IgG‐capture enzyme‐linked immunsorbent assay (GACELISA) suitable for the detection of anti‐HIV 1 and 2 in saliva and urine was developed. Following optimisation of the assay, 177 salivary and 568 urine specimens collected from individuals of known serostatus were investigated. The assay was 100% sensitive on 50 salivary (median OD/CO = 8.9) and 126 urinary (median OD/CO = 8.6) specimens collected from anti‐HIV‐positive patients. The specificity was 100% on 127 salivary specimens (median OD/CO = 0.37) and 422 urinary specimens (median OD/CO = 0.39) collected from anti‐HIV‐negative individuals. These findings demonstrate that GACELISA HIV 1 + 2 tests on saliva or on urine are an accurate alternative to a conventional anti‐HIV test of blood. This assay is satisfactory for surveillance purposes and, with appropriate precautions, could be used clinically.

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