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Novel alleles of the Escherichia coli dnaA gene

โœ Scribed by Mark D Sutton; Jon M Kaguni


Book ID
115628081
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
312 KB
Volume
271
Category
Article
ISSN
0022-2836

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The defects of temperature-sensitive dnaA and dnaN mutants of Escherichia coli are complemented by a recombinant lambda phage, which carries the bacterial DNA segment composed of two EcoRI segments of 1.0 and 3.3 kilobases. Derivatives of the phage, which have an insertion segment of Tn3 in the dnaA

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A specialized transducing lambda phage carrying the dnaN and dnaA genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respective