Novel alleles of the Escherichia coli dnaA gene
โ Scribed by Mark D Sutton; Jon M Kaguni
- Book ID
- 115628081
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 312 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0022-2836
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The defects of temperature-sensitive dnaA and dnaN mutants of Escherichia coli are complemented by a recombinant lambda phage, which carries the bacterial DNA segment composed of two EcoRI segments of 1.0 and 3.3 kilobases. Derivatives of the phage, which have an insertion segment of Tn3 in the dnaA
A specialized transducing lambda phage carrying the dnaN and dnaA genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respective