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Identification of the dnaA and dnaN gene products of Escherichia coli

✍ Scribed by Yuasa, Satoshi ;Sakakibara, Yoshimasa

Publisher: Springer
Year: 1980
Tongue: English
Weight: 686 KB
Volume: 180
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425838

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✦ Synopsis

A specialized transducing lambda phage carrying the dnaN and dnaA genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respectively. Thus the dnaN gene product was identified as a weakly acidic 41 kd protein, and the dnaA gene product as a weakly basic 48 kd protein. The synthesis of the dnaN gene product is greatly reduced by insertion of a transposon Tn3 in the dnaA gene and by deletion in the gene at the distal end to the dnaN gene. Temperature-sensitive dnaA mutations, or.L the other hand, do not affect the synthesis of the &~aN gene product. These results indicate that the synthesis of the dnaN gene product is dependent on the structural integrity of the dnaA gene.

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