✦ LIBER ✦

Norepinephrine modulates the growth-inhibitory effect of transforming growth factor-beta in primary rat hepatocyte cultures

✍ Scribed by Keith A. Houck; Jennifer L. Cruise; George Michalopoulos

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 565 KB
Volume: 135
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041350327

No coin nor oath required. For personal study only.

✦ Synopsis

TGF-beta is a potent inhibitor of EGF-induced DNA synthesis in primary rat hepatocyte cultures. Norepinephrine (NE) was shown to modulate this inhibition of DNA synthesis. It produced a five-fold increase, from 2.8 pM to 14.4 pM, in the IDSo for TGF-beta. The effect was dose-dependent and was significant at concentrations of IO-"M NE and greater. The modulation by NE was mediated by the alpha,-adrenergic receptor as shown by the ability of the alpha, antagonist prazosin to block the activity. This effect might be important during liver regeneration in allowing escape of hepatocytes from negative growth control exerted by TGF-beta.

📜 SIMILAR VOLUMES

Effects of hepatocyte growth factor on t

Effects of hepatocyte growth factor on the growth and metabolism of human hepatocytes in primary culture

✍ María José Gómez-Lechón; José Castelli; Isabel Guillén; Enrique o'Connor; Toshik 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 836 KB

The effect of recombinant human hepatocyte growth factor (h-rHGF), a potent mitogen for hepatocytes, was investigated in primary cultures of human hepatocytes. Here, we describe a series of experiments to investigate the kinetics of its mitogenic action, as well as its metabolic effects on cultured

Noradrenergic modulation of albumin expr

Noradrenergic modulation of albumin expression in growth-stimulated adult rat hepatocytes in primary culture

✍ Isabel Fabregat; Carmen De Juan; CÉSar Roncero; Manuel Benito 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 603 KB

Serum albumin is the most abundant protein synthesized by liver cells, and its production is a reliable indicator of the differentiated state of hepatocytes. We have recently shown that fetal rat hepatocytes cultured under proliferative conditions, i.e., in the presence of EGF, responded to glucagon

Effect of 2% dimethyl sulfoxide on the m

Effect of 2% dimethyl sulfoxide on the mitogenic properties of epidermal growth factor and hepatocyte growth factor in primary hepatocyte culture

✍ Diane P. Kost; George K. Michalopoulos 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 808 KB

Collagenase pretreatment and the mitogen

Collagenase pretreatment and the mitogenic effects of hepatocyte growth factor and transforming growth factor-α in adult rat liver

✍ Meng-Lun Liu; Wendy M. Mars; Reza Zarnegar; George K. Michalopoulos 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 776 KB

Hepatocyte growth factor and transforming growth factor-a are two well-known hepatomitogens for primary hepatocyte cultures. Here we report that these two growth factors also stimulate in uiuo DNA syntheses in normal, unoperated, adult rat liver after 24-hr continuous intraportal infusion. As determ

Growth of normal human hepatocytes in pr

Growth of normal human hepatocytes in primary culture: Effect of hormones and growth factors on DNA synthesis

✍ Tariq Ismail; John Howl; Mark Wheatley; Paul McMaster; James M. Neuberger; Alast 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 800 KB

Although the ability of hormones and growth factors to stimulate DNA synthesis in rat hepatocytes has been investigated extensively, no such study of human hepatocytes has been reported. Here we describe a series of experiments to identify those factors that regulate human hepatocyte DNA synthesis i

Alteration in growth regulation of hepat

Alteration in growth regulation of hepatocytes in primary culture obtained from cirrhotic rat: Poor response to transforming growth factor-β1 and interferons

✍ Shinichi Kiso; Sumio Kawata; Shinji Tamura; Nobuyuki Ito; Kenji Takaishi; Yasuhi 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 671 KB