𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Collagenase pretreatment and the mitogenic effects of hepatocyte growth factor and transforming growth factor-α in adult rat liver

✍ Scribed by Meng-Lun Liu; Wendy M. Mars; Reza Zarnegar; George K. Michalopoulos

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
776 KB
Volume
19
Category
Article
ISSN
0270-9139

No coin nor oath required. For personal study only.

✦ Synopsis

Hepatocyte growth factor and transforming growth factor-a are two well-known hepatomitogens for primary hepatocyte cultures. Here we report that these two growth factors also stimulate in uiuo DNA syntheses in normal, unoperated, adult rat liver after 24-hr continuous intraportal infusion. As determined by an immunohistochemical staining technique, 5- bromo-2'-deoxyuridine incorporation was increased in a dose-dependent fashion after infusion of up to 10 pg of growth factor/100 g m body weight in the rat. Stimulation of DNA synthesis was seen in the periportal area.

Pretreatment using intraportal infusion of collagenase

(1 U/kg body weight) for 4 hr before administration of growth factor increased the labeling by 2-to 4-fold to a labeling index range of 48% to 52%. These results suggest that collagenases and possibly other proteases are involved in making hepatocytes competent to respond to growth factors at very early stages of liver regeneration. (HEPATOLOGY 1994; 19: 1521-1527.)

Hepatocyte growth factor (HGF) and transforming growth factor-alpha (TGF-a) are two well-characterized hepatomitogens for primary hepatocyte cultures. They are both reported to be primary mitogens of liver during regeneration after partial hepatectomy or administration of CCl, (1, 2). HGF is a heparin-binding heterodimeric protein with a relative molecular weight of 97 kDa. Its primary target cells are mainly of epithelial origin, including renal epithelial cells, skin keratinocytes, gastric epithelial cells, nonparenchymal epithelial liver cells and, of course, the hepatocyte (3-9). The mitogenicity of HGF in primary hepatocyte culture is 10 times more potent on a molar basis than a TGF-arelated protein, epidermal growth factor (EGF) (10). The c-met proto-oncogene product, which displays tyrosine-base activity, has been demonstrated to be the

📜 SIMILAR VOLUMES

Expression of hepatocyte growth factor,
Expression of hepatocyte growth factor, transforming growth factor α, and transforming growth factor β 1 messenger RNA in various human liver diseases and correlation with hepatocyte proliferation
✍ M Masuhara; M Yasunaga; K Tanigawa; F Tamura; S Yamashita; I Sakaida; K Okita 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB

After a partial hepatectomy, TGF-a mRNA has been shown of chronic liver disease; CH, chronic hepatitis; LC, liver cirrhosis; LI, labeling index; RTto increase in hepatocytes at the time of DNA synthesis con-PCR, reverse-transcriptase polymerase chain reaction; cDNA, complementary DNA.

Mitogenic effects of platelet-derived gr
Mitogenic effects of platelet-derived growth factor, fibroblast growth factor, transforming growth factor-β, and heparin-binding serum factor for adult mouse Schwann cells
✍ K. Watabe; T. Fukuda; J. Tanaka; K. Toyohara; O. Sakai 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 956 KB

Mitogenic effects of fetal calf serum (FCS), plateletderived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor-p (TGF-P), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for SlOO and BrdU.

Differential expression of hepatocyte gr
Differential expression of hepatocyte growth factor, transforming growth factor-α and transforming growth factor-β1 messenger RNAs in two experimental models of liver cell proliferation
✍ Masaaki Masuhara; Sikandar L. Katyal; Toshikazu Nakamura; Hisashi Shinozuka 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 1021 KB

Hepatocyte growth factor, a potent hepatocyte mitogen in vitro, appears to trigger hepatocyte regeneration after partial hepatectomy and after acute liver cell netcrosis. Transforming growth factor-a and transforming growth factor+, may also be involved in the control of liver regeneration. In this

Modifications of the hepatocyte growth f
Modifications of the hepatocyte growth factor/c-met pathway by constitutive expression of transforming growth factor-α in rat liver epithelial cells
✍ Sharon C. Presnell; Donna B. Stolz; Wendy M. Mars; Minji Jo; George K. Michalopo 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 368 KB 👁 2 views

We have previously shown that rat liver epithelial cells (RLEC) transfected with and constitutively expressing transforming growth factor-α (TGF-α) have an enhanced mitogenic response to hepatocyte growth factor (HGF). In the study reported here, we examined tumor clones derived from the TGF-α trans

Receptor-mediated effects on ligand avai
Receptor-mediated effects on ligand availability influence relative mitogenic potencies of epidermal growth factor and transforming growth factor α
✍ Cartikeya C. Reddy; Alan Wells; Douglas A. Lauffenburger 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 639 KB

formed cells, it is also produced by a variety of normal cells in mammals, suggesting an important physiological role for TGFa (reviewed by Derynck, 1992). Human EGF and TGFa share 42% amino acid sequence homol-0 1996 WILEY-LISS, INC. ogy (Carpenter and Wahl, 1990) and are believed to exhibit simila

Levels of transforming growth factor β a
Levels of transforming growth factor β and transforming growth factor β receptors in rat liver during growth, regression by apoptosis and neoplasia
✍ Bettina Grasl-Kraupp; Walter Rossmanith; Branislav Ruttkay-Nedecky; Leonhard Mül 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 500 KB

Transforming growth factor β 1 (TGF-β 1 ) has been implicated as inhibitor of cell proliferation and a potent inducer of apoptosis in vitro and in vivo after the administration of high doses. To assess the role of endogenous TGF-β 1 , we quantitated the cytokine and its receptors in rat liver during