Nonspecific binding of the repressors CI and Cro of bacteriophage
โ Scribed by Audun Bakk; Ralf Metzler
- Book ID
- 108196015
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 300 KB
- Volume
- 231
- Category
- Article
- ISSN
- 0022-5193
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In a continuing effort to understand the molecular basis of the dimerization-coupled DNA-binding activity of the bacteriophage repressor protein, we analyzed results from conformational energy computations on the wild-type repressor protein and its mutants. We find that the hydrogen bonds between th
The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively,
By mutagenizing a 2cIts (2ci857) lysogen, a 2 mutant has been isolated with a wild-type phenotype. This mutant phage lysogenizes with low efficiency and produces a low burst. Though the initial rates of repressor synthesis in Escherichia coli after infection with wild-type and mutant 2 are the same,
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