Nondenaturing Chemical Proteomics for Protein Complex Isolation and Identification
✍ Scribed by Dr. Ghyslain Budin; Dr. Martin Moune-Dimala; Geoffray Leriche; Dr. Jean-Michel Saliou; Julie Papillon; Dr. Sarah Sanglier-Cianférani; Prof. Alain Van Dorsselaer; Dr. Valérie Lamour ; Dr. Laurent Brino; Dr. Alain Wagner
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 251 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1439-4227
No coin nor oath required. For personal study only.
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## Abstract For Abstract see ChemInform Abstract in Full Text.
The identification of individual protein species within an organism's proteome has been optimised by increasing the information produced from mass spectral analysis through the chemical derivatisation of tryptic peptides and the development of new software tools. Peptide fragments are subjected to t
A substantial degree of complexity is conferred upon the proteome by the posttranslational modification of proteins. One of the hallmarks of the proteome is its ability to dynamically regulate protein expression in response to internal and external stress. The stress can be physiological, pathologic
Peptide mass fingerprinting (PMF) remains the most amenable technique for protein identification in proteomics, using mass spectrometry as the primary analytical technique coupled with bioinformatics. This relies on the presence of the amino acid sequence of the protein in the current databanks. Des