Redox Proteomics (From Protein Modifications to Cellular Dysfunction and Diseases) || MudPIT (Multidimensional Protein Identification Technology) for Identification of Post-Translational Protein Modifications in Complex Biological Mixtures

✍ Scribed by Dalle-Donne, Isabella; Scaloni, Andrea; Butterfield, D. Allan

• Publisher: John Wiley & Sons, Inc.
• Year: 2006
• Tongue: English
• Weight: 325 KB
• Edition: 1
• Category: Article
• ISBN: 0471723452
• DOI: 10.1002/0471973122.ch8

✦ Synopsis

A substantial degree of complexity is conferred upon the proteome by the posttranslational modification of proteins. One of the hallmarks of the proteome is its ability to dynamically regulate protein expression in response to internal and external stress. The stress can be physiological, pathological, developmental, pharmacological, or aging related, and often results in the formation of various protein post-translational modifications. Proteomic analyses of expressed cellular components that are post-translationally modified will significantly help clarify the dynamics of oxidative stress. Currently, more than one hundred types of post-translational modifications are known, including glycosylation, ubiquitination, phosphorylation, oxidation, and acetylation (O'Donovan et al., 2001). Of particular interest is the oxidative damage of proteins in human diseases-a modification that leads to aberrant biological functions and to cell death.