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Nonconventional Protease Catalysis in Frozen Aqueous Solutions

✍ Scribed by Marion Hänsler; Hans-Dieter Jakubke

Book ID
105360465
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
929 KB
Volume
2
Category
Article
ISSN
1075-2617

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✦ Synopsis

During the past decade proteases have been widely used as catalysts in peptide synthesis. Unfortunately, they are not ideal ligases. Enzymatic peptide synthesis in frozen aqueous systems has been developed as an approach towards the suppression of competitive reactions. This paper summarizes reports concerning the behaviour of non-enzymatic as well as of enzyme-catalysed reactions when the reaction mixture is frozen. The advantages of freezing the reaction mixture in serine and cysteine protease-catalysed peptide synthesis, the influence of modified reaction conditions and the possible reasons for the yield-increasing effect of freezing are discussed.

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