✦ LIBER ✦

Noncompetitive enzyme immunoassay for the measurement of bronchial inhibitor in biological fluids

✍ Scribed by J.M. Tournier; J. Jacquot; P. Sadoul; J.G. Bieth

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 419 KB
Volume: 131
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90181-1

No coin nor oath required. For personal study only.

✦ Synopsis

An enzyme-linked immunosorbent assay (ELISA) of bronchial inhibitor using rabbit antibronchial inhibitor antibody-coated polystyrene balls as the solid-phase antibody and peroxidaselabeled antibody as the conjugate is described. A crude antibody fraction is used for coating the solid phase. The assay can be run within 8 h and gives reproducible results in the range of 6 to 60 rig/l of bronchial inhibitor (mean within-run coefficient of variation, 7%). It can detect bronchial inhibitor concentrations as low as 2 &I (lo-" M) and recovery of varying amounts of bronchial inhibitor added to bronchial liquids is greater than 90%. This enzyme immunoassay appears to be a convenient way to quantify bronchial inhibitor in biological fluids such as serum, sputum, or bronchoalveolar lavage fluid.

📜 SIMILAR VOLUMES

A noncompetitive enzyme immunoassay (het

A noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for salmon calcitonin: Determination of the bioavailability of subcutaneous salmon calcitonin and its correlation with the hypocalcemic activity in rats

✍ Takeyuki Kohno; Hiromichi Nakamuta; Masaki Ichikawa; Kazuhito Watabe; Masao Koid 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 645 KB

A noncompetitive enzyme immunoassay method (hetero-two-site enzyme immunoassay) for salmon calcitonin (SCT) and its usability for the pharmacokinetic study are described. The method in brief proceeds as follows: centrifugal filtration through a polysaccharide membrane to remove plasma proteins, biot

Development of an enzyme-immunoassay (EI

Development of an enzyme-immunoassay (EIA) for the measurement of follicle-stimulating-hormone (FSH) in callitrichid primates using a monoclonal antibody against the human-FSH-β-subunit

✍ J. Rosenbusch; J. A. Dias; J. K. Hodges 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 2 views

Despite the importance of Callitrichid primates in both biomedical and conservation research, practical and reliable immunoassays for the measurement of follicle-stimulating hormone (FSH) have not yet been described. A panel of monoclonal antibodies against specific peptide fragments within either t

An improved method for the measurement o

An improved method for the measurement of the angiotensin-converting enzyme inhibitor lisinopril in human plasma by stable isotope dilution gas chromatography/negative ion chemical ionization mass spectrometry

✍ H. J. Leis; G. Fauler; G. Raspotnig; W. Windischhofer 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 40 KB 👁 2 views

An improved method for the quantitative measurement of the angiotensin-converting enzyme inhibitor lisinopril in human plasma is presented. The assay is based on gas chromatography/negative ion chemical ionization mass spectrometry. The method involves solid phase extraction on C18 sorbent and deriv