β Scribed by Glenmore Shearer; Joe C. Hubbard; Howard W. Larsh
No coin nor oath required. For personal study only.
Incorporation of thymidine, thymidine monophosphate (TMP), thymidine triphosphate (TTP), uridine and orotic acid into DNA, RNA and protein in Blastomyces dermatitidis and Histoplasma capsulatum was studied utilizing a specific acid hydrolysis technique developed for these fungi. Thymidine was incorporated to the greatest extent (approximately 0.5 '),, of added label) followed by uridine, orotic acid, TMP and TTP. In Blastomyces, uridine and orotic acid labeled primarily RNA. TMP and TTP labeled RNA, DNA and protein at nearly the same level. In Histoplasma RNA was labeled poorly by any of these precursors. TMP and TTP labeled DNA predominately and protein to a slightly lower level. Deoxyadenosine or uridine media supplements of 250 pg/ml did not enhance incorporation. All precursors tested were found to be nonspecific in that RNA, DNA and protein were labeled. All data indicate that neither RNA nor DNA synthesis can be specifically measured in whole cells or acid precipitates by any of these precursors. Specific radiometric monitoring with these isotopes tberefore requires the separation of these macromolecules.
π SIMILAR VOLUMES
## Abstract Mitotic activity and DNA and RNA precursor incorporation were compared in innervated regenerating limbs and in denervated, nonregenerating limbs on days 8 and 9 postβamputation. Innervated limbs had wellβdeveloped cone stage blastemas which showed high cellular mitotic indices and H^3^β