Mitotic activity and nucleic acid precursor incorporation in denervated and innervated limb stumps of axolotl larvae
✍ Scribed by Tassava, Roy A. ;Mescher, Anthony L.
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 1014 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Mitotic activity and DNA and RNA precursor incorporation were compared in innervated regenerating limbs and in denervated, nonregenerating limbs on days 8 and 9 post‐amputation. Innervated limbs had well‐developed cone stage blastemas which showed high cellular mitotic indices and H^3^‐thymidine labeling indices of 0.40–0.50 and H^3^‐uridine labeling indices of 0.50–0.75.
In contrast, denervated limbs showed dedifferentiated cells distally under thickened wound epithelia, but essentially no mitotic activity and no blastema formation. These dedifferentiated cells showed lower levels of H^3^‐thymidine (0.10 index) and H^3^‐uridine (0.50) incorporation than regenerating limbs.
Labeling indices of wound epithelia are also compared.