Non-radioactive nucleic acid probes for the diagnosis of virus infections

✍ Scribed by H. G. Pereira

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 462 KB
• Volume: 4
• Category: Article
• ISSN: 0265-9247
• DOI: 10.1002/bies.950040305

✦ Synopsis

Nucleic acid hybridization is being increasingly used in viral diagnosis. Most of the assays described so far for this purpose require the use of radioactive probes. Their replacement by *adioactive assays has many advantages and makes the technique feasible in routine diagnostic work. Non-radioactive assays have had limited use but their diagnostic value has been demonstrated for a number of virus infections. They have the main advantages of employing stable probes, of avoiding safety hazards and of being easy and rapid to perform with limited laboratory facilities. Their main disadvantage compared to radioactive assays is in having relatively low sensitivity. Improvements in probe construction, in labelling and detecting methods, in sample treatment and in hybridization conditions may well increase their usefulness.