Non-obese-diabetic mice: immune mechanisms of pancreaticβ-cell destruction

✍ Scribed by Y. Kanazawa; K. Komeda; S. Sato; S. Mori; K. Akanuma; F. Takaku

• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 918 KB
• Volume: 27
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00275663

✦ Synopsis

The sequence of events shortly before the initiation of diabetes in female non-obese diabetic mice was studied. Immunologically, anti-lymphocyte antibodies appeared most frequently at 3 weeks of age and decreased thereafter. Insulin concentrations dropped after the initiation of mononuclear cell infiltration into the islets. The majority of female mice lost approximately 85% of their insulin at aged 22 weeks. Islet cell surface antibodies appeared most frequently during this period (12-18 weeks). Morphological examination revealed that mononuclear cells start to infiltrate islets at 6 weeks of age and involve major areas of the islets in females aged 22 weeks. Among these mononuclear cells, IgM-positive cells were found to be a major constituent, forming follicular (nodular) cell aggregates. T-helper and/or T-cytotoxic cells (Lyt-1-, and/or Lyt-2-positive cells) were fewer and located mainly around the follicular structures. Asialo GM 1-positive lymphocytes (natural killer cells), though present, were far fewer. The process of destruction of pancreatic islets in non-obese diabetic mice is discussed with emphasis on the characteristic local immune response in the pancreas.