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✦   LIBER   ✦

Non-invasive multiphoton imaging of extracellular matrix structures

✍ Scribed by Katja Schenke-Layland

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
637 KB
Volume
1
Category
Article
ISSN
1864-063X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Multiphoton microscopy has become a powerful method for the artifact‐free, nondestructive evaluation of deep‐tissue cells and extracellular matrix (ECM) structures in their native environment. By interacting with highly non‐centrosymmetric molecular assemblies such as fibrillar collagen, the non‐linear process called second harmonic generation (SHG) has also proven to be an important diagnostic tool for the visualization of ECM compartments in situ with submicron resolution without the need for tissue processing. This review reports on applications of multiphoton‐induced autofluorescence and SHG microscopy to identify collagen and elastic fiber orientation in native, tissue‐engineered and processed, as well as healthy and diseased, tissues and organs. SHG signal profiling was used to quantify ECM damage in various cardiovascular and exocrine tissues, as well as cartilage. These novel imaging modalities open the general possibility of high‐resolution in situ and more important in vivo imaging of ECM structures, cells and intracellular organelles in living intact tissues. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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