AFM Imaging of RGD Presenting Synthetic Extracellular Matrix Using Gold Nanoparticles
✍ Scribed by Susan X. Hsiong; Peter H. Cooke; Hyun-Joon Kong; Marshall L. Fishman; Maria Ericsson; David J. Mooney
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 469 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Several high‐resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High‐resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin‐labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.
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