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No effect of guanidine on an improved in-vitro system for viral RNA polymerase

โœ Scribed by Klaus Koschel

Book ID
118850987
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
322 KB
Volume
37
Category
Article
ISSN
0006-291X

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โœ Young, Barbara S. ;Wright, Andrew ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 585 KB

S. typhimurium strain BY324 is temperature sensitive due to a mutation (rpo C32) in the gene for the RNA polymerase fi' subunit. Transcription of T7 DNA by RNA polymerase purified from this strain is temperature sensitive in vitro. The enzyme is slightly defective in template binding and RNA chain i