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NMR spectroscopic characterization of the membrane affinity of polyols

✍ Scribed by Daniela Fischer; Armin Geyer

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
177 KB
Volume
43
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

Residual dipolar couplings (RDCs) are applied here for the analysis of weak, transient binding events between phosphatidylcholine bilayers and polyols. Large signal responses are observed even for low percentages of ‘ligand‐receptor complexes,’ making RDCs a sensitive tool for the analysis of molecular recognition events. The different degree of alignment in solution can be compared as a result of the calculation of the alignment tensor elements. By varying polarity and/or charge of the molecules under investigation, nonspecific hydrophobic effects can be excluded. Copyright © 2005 John Wiley & Sons, Ltd.

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