✦ LIBER ✦

NMR reinvestigation of two N-acetylneuraminic acid-containing O-specific polysaccharides (056 and 024) of Escherichia coli

✍ Scribed by Vladimir I. Torgov; Alexander S. Shashkov; Barbara Jann; Klaus Jann

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 708 KB
Volume: 272
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(95)00041-q

No coin nor oath required. For personal study only.

✦ Synopsis

Structures for the N-acetylneuraminic acid (Neu5Ac)-containing 056 and 024 polysaccharides of Escherichia coli have been reported previously. During these studies unusual chemical shifts had been observed for the NMR signals for H-3eq and C-3 of the Neu5Ac residues of both polysaccharides. In further pursuing this phenomenon, we have reinvestigated the 056 and 024 polysaccharides as well as derived oligosaccharides by one-and two-dimensional NMR spectroscopy. The results showed that structures of both polysaccharides (PSs) had to be modified and formulated as

2D ROESY spectra revealed a strong NOE between H-3eq of NeuSAc and the protons of the side-chain sugar (H-3 and H-5 of ~-o-Galp in the 056 PS and H-3 of Ot-D-Glcp in the 024 PS) and also between H-3ax of Neu5Ac and H-3 of fl-D-Glcp in the main chain. This indicated a

📜 SIMILAR VOLUMES

Structure of the O56 antigen of Escheric

Structure of the O56 antigen of Escherichia coli, a polysaccharide containing 7-substituted α-N-acetylneuraminic acid

✍ Grigorij Kogan; Alexander S. Shashkov; Barbara Jann; Klaus Jann 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 616 KB

The 056 polysaccharide moiety of the 056 antigen (LPS) consists of D-glucose, o-galactose, 2-acetamido-2-deoxy-o-glucose, and N-acetylneuraminic acid in the molar ratios 1: 1: 1: 1. Methylation analysis, periodate oxidation, mild acid hydrolysis, as well as 'H and 13C NMR spectroscopy showed that th

The structure of the sialic acid-contain

The structure of the sialic acid-containing Escherichia coli O104 O-specific polysaccharide and its linkage to the core region in lipopolysaccharide

✍ Andrzej Gamian; Elzbieta Romanowska; Jacques Ulrich; Jacques Defaye 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 924 KB

Mild acid hydrolysis of Escherichia coli O104 lipopolysaccharide released an O-specific polysaccharide, a tetrasaccharide repeating unit, the corresponding dimer, and a disaccharide fragment of the repeating unit. Complete and incomplete cores, and oligosaccharides comprising fragments of the repeat