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NMR Evidence for the Participation of a Low-Barrier Hydrogen Bond in the Mechanism of Δ5-3-Ketosteroid isomerase

✍ Scribed by Qinjian Zhao, Chitrananda Abeygunawardana, Paul Talalay and Albert S. Mildvan


Book ID
123640018
Publisher
National Academy of Sciences
Year
1996
Tongue
English
Weight
935 KB
Volume
93
Category
Article
ISSN
0027-8424

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Using low-temperature UV, 1 H and 15 N NMR spectroscopy, we studied the easily polarizable bond of the labeled Mannich base[ 15 N] 2-(N,Ndiethylaminomethyl)-3,4,6-trichlorophenol (Cl 3 MB) dissolved in dichloromethane and in a 2 : 1 mixture of CDF 3 and CDClF 2 . Whereas at high temperature the mol