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NMR assignment of the N-terminal region of human La free and in complex with RNA

✍ Scribed by Domenico Sanfelice; Geoff Kelly; Stephen Curry; Maria R. Conte


Book ID
107599439
Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
253 KB
Volume
2
Category
Article
ISSN
1874-2718

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The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton resonances in a uniformly 15 N-enriched macromolecule involved in a complex, starting from the free form assignments, are demonstrated on a protein-DNA complex. This 2D experiment is