✦ LIBER ✦
New 15N NMR Exchange Experiments for the Unambiguous Assignment of 1HN/15N Resonances of Proteins in Complexes in Slow Chemical Exchange with Free Form
✍ Scribed by Catherine Vialle-Printems; Carine van Heijenoort; Eric Guittet
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 120 KB
- Volume
- 142
- Category
- Article
- ISSN
- 1090-7807
No coin nor oath required. For personal study only.
✦ Synopsis
The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton resonances in a uniformly 15 N-enriched macromolecule involved in a complex, starting from the free form assignments, are demonstrated on a protein-DNA complex. This 2D experiment is further extended to a 3D experiment in the case of severe superpositions.