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NMR analysis of the O-antigen polysaccharide from Escherichia coli strain F171

✍ Scribed by Johan Fundin; Andrej Weintraub; Jian-Guo Xu; Göran Widmalm

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
157 KB
Volume
41
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

The structure of the O‐antigen polysaccharide from Escherichia coli strain F171 has been determined. NMR analysis of the polysaccharide showed that it was composed of pentasaccharide repeating units. The ^1^H and ^13^C signals were assigned by 2D NMR techniques which revealed severe spectral overlap for key resonances at substitution positions. The structure of the repeating unit was deduced from ^1^H,^13^C HMBC and, in particular, by ^1^H,^1^H NOESY experiments as follows:

The structure is identical with that of the O‐antigen from Escherichia coli O25 previously determined by chemical degradation methods and the strain F171 should therefore belong to this serogroup. Copyright © 2003 John Wiley & Sons, Ltd.

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