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Nitroimidazole derivatives: non-randomness sister chromatid exchanges in human peripheral blood lymphocytes

✍ Scribed by Marta Ana Carballo; Romari Alejandra Martinez; Marta Dolores Mudry

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
198 KB
Volume
29
Category
Article
ISSN
0260-437X

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✦ Synopsis

Abstract

Nitroimidazole derivatives exhibited genotoxic effect in different experimental conditions. This study focuses on an evaluation of possible genomic targets, at a chromosomal level, of two 5‐nitroimidazoles (ornidazole and metronidazole) using the in vitro human peripheral blood culture as experimental system. We observed that both derivatives showed a decrease in mitotic index (MI) (P < 0.001), an increase in sister chromatid exchanges (SCE) frequency (P < 0.001) and no modifications in cellular proliferation kinetics (CPK). As a null hypothesis we considered the assumption that larger chromosomes should harbor more SCE, which was viewed using a novel sequential G‐band (400 band resolution)/SCE technique. The analysis showed highly significant chi square values (P < 0.001), indicating that SCE frequency per chromosome is not proportional to chromosome length. SCE could be considered an instability indicator due to the high correlation between SCEs in certain chromosomal bands and the exposure to nitroimidazole derivatives. Copyright Β© 2008 John Wiley & Sons, Ltd.

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