Nitrogen regulation of avermectins biosynthesis in Streptomyces avermitilis in a chemically defined medium

✍ Scribed by E. Cimburková; J. Zima; J. Novák; Z. Vanék

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 436 KB
• Volume: 28
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620280805

✦ Synopsis

The biosynthesis of avermectins was significantly affected by the nitrogen source used. The maximum yield of AVMs') was achieved in a chemically defined medium with ammonium sulfate as the sole nitrogen source. Higher concentrations of ammonia inhibited the production of AVMs without the inhibition of the growth. AVMs of the group B were prevailing in the complex until I5 mM ammonium sulfate. At higher concentrations, AVMs of the group A predominated. Of various amino acids tested, lysine and asparagine were found to be the best for the biosynthesis of AVMs. The concentration of most amino acids higher than 0.2% inhibited the production, while the mycelium was still growing. The biosynthesis of AVMs was completely suppressed by 15.2 m~ isoleucine, however, this inhibition was reversed by adding alanine at the beginning of cultivation.