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Nitrogen Fixation in Wheat Provided by Klebsiella pneumoniae 342

✍ Scribed by Iniguez, A. Leonardo; Dong, Yuemei; Triplett, Eric W.


Book ID
120996218
Publisher
The American Phytopathological Society
Year
2004
Tongue
English
Weight
447 KB
Volume
17
Category
Article
ISSN
0894-0282

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Complementation analysis of Klebsiella p
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A series of mutants defective in nitrogen fixation (nif) were isolated in Klebsiella pneumoniae strain M5a1. The nif mutations were either located on plasmid pRD1 or on the K. pneumoniae chromosome. A total of 37 plasmid mutants and 28 chromosomal mutants were employed in complementation tests using

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The nif cistrons indentified by complementation analysis in the preceding paper (Dixon et al., 1977) were mapped with respect to hisD and to each other other by P1 cotransduction and three-factor reciprocal crosses. The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH. Analysis of h