𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Nitrogen-15 n.m.r. studies of 13C, 15N labeled arginine

✍ Scribed by R. E. London; T. E. Walker; T. W. Whaley; N. A. Matwiyoff

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
328 KB
Volume
9
Category
Article
ISSN
0749-1581

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

^15^N n.m.r. spectra of [^13^C‐2, 3‐^15^N~2~‐guanidino]arginine and [^13^C, ^15^N~2~] urea were obtained in D~2~O and H~2~O at a variety of pH values both with and without proton decoupling. The effects of the proton exchange rate are readily observable in the proton coupled ^15^N spectra. When the guanidino group is deprotonated (p__K__ = 12.5), the terminal nitrogens give a single resonance 6.6 ppm downfield of the protonated species, indicating a rapid tautomeric exchange. The observed NH and CN couplings are compared with calculated values, and good agreement is found for ^1^J(CN) using a Blizzard–Santry type calculation. The ramifications of the proton exchange on ^15^N n.m.r. spectra of amino acids and peptides are discussed.

📜 SIMILAR VOLUMES

Syntheses of [13C,15N]-labeled polyamine
Syntheses of [13C,15N]-labeled polyamines
✍ Takeshi Hara; Yong Ji Xu; Hitomi Sasaki; Masaru Niitu; Keijiro Samejima 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 318 KB
15N13C coupling constants in 15N labelle
15N13C coupling constants in 15N labelled azaadamantane
✍ Stefan Berger; Hans Kaletsch 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB

## Abstract The ^15^N^13^C coupling constants in ^15^N labelled azaadamantane have been measured and interpreted in terms of their conformational dependence as compared with ^15^N‐quinuclidine.

Synthesis of labeled acrylamide and N-me
Synthesis of labeled acrylamide and N-methylolacrylamide (NMA): 15N-acrylamide, 13C-NMA, 15N-NMA, and 13C,15N-NMA
✍ N. R. Brown; C. E. Frazier 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 115 KB

Labeled derivatives of N-methylolacrylamide (NMA) including 15 N-NMA, 13 C-NMA, and 13 C, 15 N-NMA were synthesized and purified. A required chemical precursor, 15 N-acrylamide, was also prepared. Reported methods for synthesizing unlabeled analogs are noted, and modifications to these methods for a

15N and 13C n.m.r. study of azimines and
15N and 13C n.m.r. study of azimines and azoxybenzenes
✍ W. Schwotzer; Ch. Leuenberger; L. Hoesch; A. S. Dreiding; W. von Philipsborn 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 195 KB

## Abstract ^15^N chemical shifts, ^1^__J__(NN), ^1^__J__(NC) and ^2^__J__(NC) coupling constants have been used to prove the open chain structure and configuration of __cis__‐ and __trans__‐2,3‐diphenyl‐1‐phthalimidoazimines. For comparison, the corresponding data of the iso‐π‐electronic __cis__‐

Nuclear magnetic resonance studies: VII—
Nuclear magnetic resonance studies: VII—13C and 15N n.m.r. of diazo compounds
✍ Thomas A. Albright; Walter J. Freeman 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 469 KB

## Abstract The ^13^C and ^15^N n.m.r. results for a series of diazo compounds are reported. It is found that the diazo carbon is shielded by an extraordinary amount compared with normal sp^2^ hybridized carbons. The ^15^N chemical shifts reveal that the terminal nitrogen is deshielded relative to

Synthesis of 15N and 13C selectively lab
Synthesis of 15N and 13C selectively labeled anandamide
✍ Xiang-Qun Xie; Hongqin Yang; Greg Choi; Sunyuan Lin; Alexandros Makriyannis 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 137 KB

## Abstract Anandamide (Figure 1a) (arachidonyl ethanolamide, AEA), (5Z,8Z,11Z,14Z)‐N‐(2‐hydroxyethyl)‐5,8,11,14‐Eicosatetraenamide, is an endogenous cannabinoid ligand possessing important biological activity. The conformation of AEA in its native receptor binding environment is particularly of in