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Nitrilases and amidases: Determination of activity by proton magnetic resonance spectrometry

โœ Scribed by J.C. Jallageas; A. Arnaud; P. Galzy


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
461 KB
Volume
95
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


A rapid and easy method based on proton magnetic resonance spectrometry is proposed for the determination of nitrilase and amidase activities. The technique is applicable to many substrates of different chemical types: aliphatic, a-ethylenic, cY-hydroxy, and a-amino nitriles and amides. The rate of hydrolysis of the above substrates may be monitored in a continuous fashion by using proton magnetic resonance. A good correlation has been achieved between the results obtained by proton magnetic resonance and those found by gas-liquid chromatography.


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