A simple, accurate and specific proton nuclear magnetic resonance spectrometric method is described for the quantitative determination of atropine and scopolamine in plants. The method is based on a comparison of the integrated peak areas of the N-CH 3 protons of both compounds (centred at 2.15 and
Nitrilases and amidases: Determination of activity by proton magnetic resonance spectrometry
โ Scribed by J.C. Jallageas; A. Arnaud; P. Galzy
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 461 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A rapid and easy method based on proton magnetic resonance spectrometry is proposed for the determination of nitrilase and amidase activities. The technique is applicable to many substrates of different chemical types: aliphatic, a-ethylenic, cY-hydroxy, and a-amino nitriles and amides. The rate of hydrolysis of the above substrates may be monitored in a continuous fashion by using proton magnetic resonance. A good correlation has been achieved between the results obtained by proton magnetic resonance and those found by gas-liquid chromatography.
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