Nicotinic acid phosphoribosyltransferase activity in human erythrocytes: Studies using a new HPLC method
β Scribed by M. Pescaglini; V. Micheli; H.A. Simmonds; M. Rocchigiani; G. Pompucci
- Book ID
- 115828095
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 668 KB
- Volume
- 229
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
A radiochemical reverse-phase-high-performance liquid chromatography-linked method to measure the activity of nicotinamide phosphoribosyltransferase (EC 2.4.2.12) in crude lysates of human red blood cells is described. The apparent Km for nicotinamide was in the micromolar range, much lower than tha
A new method for the determination of adenine phosphoribosyltransferase (APRT) activity in human erythrocytes is described. APRT activity was assayed by a non-radiochemical method in which adenosine mnnophosphate (AMP) and AMY metabolites produced from a substrate adenine were converted to inosine b