New uses for molecular-size exclusion chromatography

✍ Scribed by Michael J. Telepchak

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 666 KB
• Volume: 83
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97033-x

✦ Synopsis

The discovery of new packing materials for high-speed molecular-size exclusion has brought this mode of liquid chromatography to a level of importance equal to that of the other three modes. Substantial reductions in the time of analysis, column lengths and column diameters, coupled with improved analytical results, yield considerable improvements when applied to proven methods and make possible many new applications using molecular-size exclusion chromatography as a general analytical technique.

New applications are demonstrated by actual chromatograms and include analyses such as: (1) molecular-weight distributions on 2 m x 2.6 mm I.D. columns using IO-20 ml of solvent, giving results in 20 min with =t 5 % reproducibility;

(2) fractionation of blood plasma; (3) analysis of drugs in blood plasma without the need for sample clean-up: (4) analysis of alcohol in beverages; and (5) analysis of decomposition products of drugs. Other important applications are also included.

All of these applications are carried out on OS-2 m x 2.6 mm I.D. columns using either aqueous or organic solvents. The analyses are completed in lo-20 min using IO-20 ml of solvent and all applications are quantitative. Plate counts of 1000 plates/m are normal.

The above work demonstrates that with the advent of new high-speed molecular-size exclusion packings, molecular-size exclusion takes on a new importance as a general analytical technique and should be given equal consideration when choosing the mode of operation for solving a particular separation problem.