New radiochemical method for assay of enzymes catalyzing the cleavage of inorganic pyrophosphate
โ Scribed by Jukka Heinonen
- Book ID
- 102983064
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 601 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A new method for the rapid analysis of inorganic pyrophosphate (PPJ which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5-50.0 nmol of PP, and is not affected by inorganic phosp
A simple, rapid enzymatic assay for the determination of inorganic pyrophosphate in tissue and plasma has been developed using the enzyme pyrophosphate--fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.90) which was purified from extracts of Propionibacterium shermanii. The enzyme phosphorylates